What is Tissue Culture?

Tissue culture (TC) is the cultivation of plant cells, tissues, or organs on specially formulated nutrient media. Under the right conditions, an entire plant can be regenerated from a single cell. Plant tissue culture is a technique that has been around for more than 30 years. Tissue culture is seen as an important technology for developing countries for the production of disease-free, high quality planting material and the rapid production of many uniform plants.

Micropropagation, which is a form of tissue culture, increases the amount of planting material to facilitate distribution and large scale planting. In this way, thousands of copies of a plant can be produced in a short time. Micropropagated plants are observed to establish more quickly, grow more vigorously and are taller, have a shorter and more uniform production cycle, and produce higher yields than conventional propagules.

Plant tissue culture is a straightforward technique and many developing countries have already mastered it. Its application only requires a sterile workplace, nursery, and green house, and trained manpower. Unfortunately, tissue culture is labor intensive, time consuming, and can be costly. Plants important to developing countries that have been grown in tissue culture are oil palm, plantain, pine, banana, date, eggplant, jojoba, pineapple, rubber tree, cassava, yam, sweet potato, and tomato. This application is the most commonly applied form of traditional biotechnology in Africa.


Tissue culture often requires sterile working conditions and thus is typically performed in a laminar flow cabinet (or tissue culture hood), which circulates filtered air to reduce the risk of culture contamination.

Mushroom Cultivation

Mushroom is a fleshy-fungi, it’s a small umbrela like structure. It has two part, cap like structure is known as PILEUS, attached with thread like structure is known as MYCELIA. Mycelia absorb nutrient from soil, it do not require sun light for their growth.

Edible Mushroom

✓ Agaricus bisporus
✓ Agaricus bitorquis
✓ Lentinus edodes
✓ Tremella fusiformis
✓ Flammulina velutips

Raw Material

✓ Animal excreta
✓ Animal manure
✓ Horse manure
✓ Vegetable waste
✓ Forest litters
✓ Straw from wheat/rice
✓ Wheat bran

Cultivation Method

1. Garden & field cultivation
2. Cave cultivation
3. Indoor cultivation